Micro RNA (miR) are recognized for their important roles in biological processes, particularly in regulatory componentization.Among the miR, miR-150 has been the focus of intense scrutiny, mostly due to its role in malignant tumors.A comparison between steer and bull adipose tissues identified bta-miR-150 as one of the nine downregulated miRNAs, although its function remains tanguray covington unknown (GEO:GSE75063).The present study aimed to further characterize the role of bta-miR-150 in cattle.
bta-miR-150 has a negative regulatory effect on the differentiation of bovine adipocytes and promotes proliferation.Overexpression of bta-miR-150 can promote mRNA and protein expression of the marker genes CDK1, CDK2, and PCNA, increase the number of EdU-stained cells, promote adipocyte proliferation, inhibit adipocyte differentiation, and reduce lipid droplet formation.Results of RNA-seq and WGCNA analyses showed that the mammalian target of the rapamycin signaling pathway, which plays a major regulatory role, is dysregulated by the overexpression and inhibition of miR-150.We found that the target gene of bta-miR-150 is AKT1 and that bta-miR-150 affects AKT1 phosphorylation levels.
These results showed that bta-miR-150 plays a role in adipogenic differentiation p3221d and might therefore have applications in the beef industry.